Infections caused by arboviruses such as dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) frequently occur in tropical and subtropical regions. These three viruses are transmitted by Aedes (Ae.) aegypti and Ae. albopictus. Zika (ZIKV), Dengue (DENV) and Chikungunya viruses (CHIKV) co-circulate in the same geographical areas during the same seasonal period through the same biting arthropods. Prior to the arrival and subsequent spread of Zika virus (ZIKV)in Latin America and the Caribbean, Dengue virus (DENV) was the predominant arbovirus. With the recent increase of ZIKV and Chikungunya (CHIKV) circulation worldwide, several instances of human co-infection have been reported and characterized, being co-infection with more than one DENV serotype associated to more severe forms. Therefore a rapid sensitive and specific molecular assay for these viruses would be a considerable help in the disease management and the epidemiological survey.
RNAs from serum
RNA extract 5 μL